Transcription in Prokaryotes MCQ
Ans: 1
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GTFs are of the followingtypes: MNEMONICS TO REMEMBERTRANSCRIPTION FACTOR IN SEQUENCE Dogs Barkand Bite People Frequently Every Halloween Dogs = TFIID; Bark = TBP; Bite = TFIIB; People = RNA P; Frequently = TFIIF; Every = TFIIE; Halloween= TFIIH STEPS: ELONGATION of Transcription in Eukaryotes: RNA of eukaryotes undergoes furtherprocessing before it is translated. RNA
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Q. What is gene expression? Operon Concept for Regulation of Gene Expression LAC OPERON CONCEPT
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Differences in the Process of Transcription in Prokaryotes versus Eukaryotes Transcription Unit in DNA: The conventional numbering system of promoters Structural Gene Present in the Transcription Unit: Types of RNA and the Process of Transcription Structure of RNA polymerase Enzyme: Steps of Transcription: Termination: Rho Dependent Transcription:
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Unwinding of DNA Double Helix: Addition of Primer to the DNA helix: Polymerization of nucleotides for the formation of new DNA strands: Summary of DNA polymerase enzyme: Enzyme DNA pol I DNA pol II DNA pol III 5’– 3’ polymerase Yes Yes Yes 3’ — 5’ exonuclease Yes Yes Yes 5’ — 3’ exonuclease Yes
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The three different DNA replication models suggested by scientists include: Conservative model of DNA: According to this model the parental DNA will be conserved in the next generation. For example, a cell containing a double stranded parental DNA (represented by black color) will divide by mitosis into two daughter cells. Now, one daughter cell will
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Introduction Structure of DNA The structure of DNA was first revealed through the extraction of nuclein by Meischer. Later, it was identified as nucleic acid, a polymer of nucleotides. These nucleotides are composed of phosphate, sugar, and nitrogenous bases. Watson and Crick’s model of DNA in 1953 demonstrated its double-helix form, a critical structure for
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Following experiments were done to prove that DNA was the genetic material: Griffith Experiment in Search for the Genetic Material Avery, McLeod and McCarty Experiment in Search for the Genetic Material Hershey and Chase Experiment in Search for the Genetic Material
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