MCQ BIOTECHNOLOGY AND ITS APPLICATIONS

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Q1. Which of the following factors contributed to green revolution?

  1. Use of improved crop varieties
  2. Use of agro chemicals
  3. Both a and b
  4. Only b

Ans: 3

Q2. Which of the following are the advantages of GM plants?

  1. GM crops are more tolerant to abiotic stresses (cold, draught, salt, heat)
  2. GM technology helps to reduce post harvest losses
  3. GM technology has increased efficiency of mineral usage by plants
  4. GM technology has enhanced nutritional value of food
  5. All of the above

Ans: 5

Q3. Golden rice is genetically modified rice, which is enriched in:

  1. Vitamin B
  2. Vitamin A
  3. Vitamin C
  4. Vitamin D

Ans: 2

Q4. Agrobacterium tumifaciens cause crown gall in:

  1. Dicot plants
  2. Monocot plants
  3. Both a and b
  4. Gymnosperms

Ans: 1

Q5. Which of the following is true for Bacillus thuringiensis?

  1. It is a soil bacterium
  2. This bacterium produces a toxin, which is effective against boll worm, beetles, insects and flies
  3. The bacterium produces toxin during sporulation in the form of proto-toxin
  4. The bacterial toxin is active only under alkaline condition
  5. All of the above

Ans: 5

Q6. Which of the following cry genes are responsible for controlling bollworms?

  1. cryIAb
  2. cryIAc
  3. cryIIAb
  4. cryIAc and cryIIAb

Ans: 4

Q7. Which of the following cry gene is responsible for controlling corn borer?

  1. cryIAb
  2. cryIAc
  3. cryIIAb
  4. cryIAc and cryIIAb

Ans: 1

Q8. Which of the following steps are not involved in the formation of transgene animals?

  1. Selection of desirable gene
  2. Isolation of gene and transfer to a suitable vector
  3. Transfer of recombinant vector to animal’s germ cells
  4. Transfer of recombinant vector to animal’s somatic cells

Ans: 3

Q9. Which of the following option can be harmful for increasing food production?

  1. Agro-chemical based agriculture
  2. Genetically engineered based agriculture
  3. Both a and b
  4. Organic agriculture

Ans: 1

Q10. Which of the following is the benefit of genetically modified plants?

  1. GM plants are more tolerant to abiotic stresses
  2. GM plants have increased efficiency of mineral usage
  3. GM plants possess enhanced nutritional value
  4. GM plants have reduced reliance on chemical pesticides
  5. All of the above

Ans: 5

Q11. Bacillus thuringiensis produce proteins that kill certain insects. Bt toxin protein exist as inactive protoxins, but once an insect ingest the inactive toxin, it is converted to an active toxin due to:

  1. Acidic pH of the gut
  2. Alkaline pH of the gut
  3. Neutral pH of the gut
  4. None of the above

Ans: 2

Q12. Which of the following cry genes are involved in the formation of toxin protein to control cotton bollworm?

  1. cryIAc
  2. cryIAc and cryIIAb
  3. cryIAb
  4. cryIAb and cryIIAc

Ans: 2

Q13. Which of the following cry gene is responsible for controlling corn borer pest?

  1. cryIAb
  2. cryIAc
  3. cryIIAb
  4. cryIAc and cryIIAb

Ans: 1

Q14. RNA interference (RNAi) mechanism is used to protect tobacco plant root from nematode infection. Which of the following statement is true for RNAi technique?

  1. RNAi takes place in all eukaryotic organisms as a method of cellular defense
  2. RNAi involves silencing of a specific mRNA by using dsRNA
  3. RNAi technique involves agrobacterium vector to transfer nematode-specific genes into the host plant
  4. All of the above

Ans: 4

Q15. Thirty recombinant therapeutics have been approved for human-use the world over. How many of these are presently being marketed in India?

  1. Thirty
  2. Twenty five
  3. Twenty
  4. Twelve

Ans: 4

Q16. How many disulfide bonds are found in pro-insulin?

  1. One
  2. Two
  3. Three
  4. Four

Ans: 3

Q17. How many disulfide bonds are present in insulin?

  1. One
  2. Two
  3. Three
  4. Four

Ans: 3

Q18. How many different types of polypeptide chains are involved in the formation of pro-insulin?

  1. 4
  2. 3
  3. 2
  4. 5

Ans: 2

Q19. In active insulin, the extra stretch of polypeptide chain is removed. Which of the following is that extra stretch of polypeptide?

  1. Polypeptide A
  2. Polypeptide B
  3. Polypeptide C
  4. Polypeptide D

Ans: 3

Q20. Which of the following statement is incorrect in terms of the length of polypeptides present in pro-insulin?

  1. Polypeptide A is made up of 21 amino acids
  2. Polypeptide B is made up of 30 amino acids
  3. Polypeptide C is made up of 30 amino acids
  4. Polypeptide C is made up of 51 amino acids

Ans: 4

Q21. Which of the following statement is true for pro-insulin structure?

  1. Pro-insulin is made up of three polypeptides: A, B and C
  2. Polypeptide A and B are attached by three disulfide bond
  3. Polypeptide C is attached to polypeptide B
  4. All of the above

Ans: 4

Q22. Eli Lily an American company prepared human insulin. Which of the following rDNA technique was used to form human insulin?

  1. Two genes coding for polypeptide A and B were extracted from human pancreas
  2. The two genes were integrated in plasmids of E. coli to produce insulin chains
  3. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin
  4. All of the above

Ans: 4

Q23. The first clinical gene therapy was given in 1990 to a 4-year old girl with:

  1. PKU disease
  2. ADA deficiency
  3. Sickle-cell anemia
  4. Thalassemia

Ans: 2

Q24. Other than gene therapy, what other methods are used to treat ADA deficiency disorder?

  1. Blood transfusion
  2. Bone marrow transplantation
  3. Enzyme replacement therapy
  4. Both b and c

Ans: 4

Q25. Which of the following gene therapy technique is used for the treatment of ADA deficiency disorder?

  1. A functional ADA gene is introduced into the somatic cells of the baby
  2. A functional ADA cDNA is introduced into the cultured lymphocytes and returned to the patient
  3. A functional ADA cDNA is introduced into the cultured monocytes and then returned to the patient
  4. A functional ADA cDNA is introduced into the cultured neutrophils and then returned to the patient

Ans: 2

Q26. Which of the following technique could be used for the early detection of a disease?

  1. Recombinant DNA technology (RDT)
  2. Polymerase Chain Reaction (PCR)
  3. Enzyme Linked Immuno-sorbent Assay (ELISA)
  4. All of the above

Ans: 4

Q27. Among transgenic rats, rabbits, pigs, sheep, cows, fish and mice, which of the following animal covers over 95% of all existing transgenic animals?

  1. Rats
  2. Pigs
  3. Mice
  4. Sheep

Ans: 3

Q28. For which of the following disease, transgenic models are used?

  1. Cancer
  2. Cystic fibrosis
  3. Rheumatoid arthritis
  4. Alzheimer’s disease
  5. All of the above

Ans: 5

Q29. Human protein alpha-1-antitrypsin is a biological product of the recombinant gene technology. Alpha-1-antitrypsin is used for the treatment of which of the following disease?

  1. Emphysema
  2. PKU
  3. Cystic fibrosis
  4. Sickle-cell anemia

Ans: 1

Q30. In 1997, the first transgenic cow produced human protein enriched milk (2.4g/l). The milk contained the human alpha-lactalbumin. What was the name of the first transgenic cow?

  1. Sony
  2. Rosie
  3. Myria
  4. Jasmine

Ans: 2

Q31. GEAC stands for:

  1. Genetic Engineering Approval Committee
  2. Genetic Engineering Authority Committee
  3. Genetic Engineering Address Committee
  4. Genetic Engineering Appraisal Committee

Ans: 4

Q32. Which of the following is the function of GEAC?

  1. GEAC make decisions regarding the validity of GM research and the safety of introducing GM-organisms for public services
  2. GEAC is the government body that provides funds for genetic engineering research
  3. GEAC is associated with patenting of the genetic engineering mediated products
  4. GEAC is a private organization responsible for checking the validity of GM research and the safety of introducing GM organisms for public services

Ans: 1

Q33. How many different varieties of rice are available in India?

  1. 100000
  2. 200000
  3. 50000
  4. 25000

Ans: 2

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